LOCUS       40924_35868        7237 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Targeting vector pP{white-STAR}, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7237)
  AUTHORS   Choi CM, Vilain S, Langen M, Van Kelst S, De Geest N, Yan J, 
            Verstreken P, Hassan BA.
  TITLE     Conditional mutagenesis in Drosophila
  JOURNAL   Science 324 (5923), 54 (2009)
  PUBMED    19342580
REFERENCE   2  (bases 1 to 7237)
  AUTHORS   Choi CM, Vilain S, Langen M, Van Kelst S, De Geest N, Yan J, 
            Verstreken P, Hassan BA.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2009) Department of Molecular and Developmental 
            Genetics, VIB, Herestraat 49, Leuven 3000, Belgium
REFERENCE   3  (bases 1 to 7237)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7237)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science"; 
            date: "2009"; volume: "324"; issue: "5923"; pages: "54"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-FEB-2009) Department of Molecular and Developmental Genetics, 
            VIB, Herestraat 49, Leuven 3000, Belgium"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    25..42
                     /label=I-SceI site
                     /note="I-SceI site"
     protein_bind    43..76
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     misc_feature    complement(102..687)
                     /label=P element 5' end
     rep_origin      complement(922..1510)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1684..2541)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2542..2646)
                     /label=AmpR promoter
     misc_feature    complement(2731..2963)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3026..3059
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     misc_feature    3026..3038
                     /label=FRT
                     /note="FRT"
     misc_feature    3060..3077
                     /label=I-SceI site
                     /note="I-SceI site"
     misc_feature    3104..3153
                     /label=attP
                     /note="attP"
     CDS             join(3646..3717,4113..4385,4460..5110,5176..5490,5714..5843,
                     5914..6527)
                     /codon_start=1
                     /gene="white"
                     /product="white"
                     /label=white
                     /protein_id="ACN64944.1"
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNVTADASQSCINQGFGQAKNYG
                     TLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERHI
                     PAPRKHLLKNGCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNGQ
                     PVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQELS
                     LSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQV
                     LKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSWVPSVLPT
                     TIRRTFTYRCWPLCPDGRSSPVIGSPRYGDNFAISKVARDMEQLLATKNLEKPLEQPEN
                     GYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTVAILIGLIFLGQQLTQVGVMN
                     INGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPLFLT
                     VPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSVGPP
                     VIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSSNTT
                     CPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     gene            3646..6527
                     /gene="white"
                     /label=white
     misc_feature    complement(7186..7235)
                     /label=attP
                     /note="attP"
ORIGIN
        1 ggccgctata actagtaact cgagtaggga taacagggta atgaagttcc tattctctag
       61 aaagtatagg aacttcggat ccaaacctag taattaattc ggctgctgct ctaaacgacg
      121 catttcgtac tccaaagtac gaattttttc cctcaagctc ttattttcat taaacaatga
      181 acaggaccta acgcacagtc acgttattgt ttacataaat gatttttttt actattcaaa
      241 cttactctgt ttgtgtactc ccactggtat agccttcttt tatcttttct ggttcaggct
      301 ctatcacttt actaggtacg gcatctgcgt tgagtcgcct ccttttaaat gtctgacctt
      361 ttgcaggtgc agccttccac tgcgaatcat taaagtgggt atcacaaatt tgggagtttt
      421 caccaaggct gcacccaagg ctctgctccc acaattttct cttaatagca cacttcggca
      481 cgtgaattaa ttttactcca gtcacagctt tgcagcaaaa tttgcaatat ttcatttttt
      541 tttattccac gtaagggtta atgttttcaa aaaaaaattc gtccgcacac aacctttcct
      601 ctcaacaagc aaacgtgcac tgaatttaag tgtatacttc ggtaagcttc ggctttcgac
      661 gggaccacct tatgttattt catcatgggc cagacccacg tagtccagcg gcagatcggc
      721 ggcggagaag ttaagcgtct ccaggatgac cttgcccgaa ctggggcacg tggtgttcga
      781 cgatgtgcag ctaatttcgc ccggctccac gtccgcccat tggttaatca gcagaccctc
      841 gttggcgtaa cggaaccatg agaggtacga caaccatttg aggtatactg gcaccgagcc
      901 cgagttcaag aagaaggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
      961 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     1021 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     1081 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     1141 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     1201 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     1261 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     1321 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     1381 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     1441 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     1501 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     1561 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     1621 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     1681 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     1741 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     1801 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     1861 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     1921 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     1981 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     2041 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     2101 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     2161 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     2221 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     2281 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     2341 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     2401 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     2461 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     2521 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     2581 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     2641 tccgcgcaca tttccccgaa aagtgccacc tgcgaagtct tagagccaga tatgcgagca
     2701 cccggaagct cacgatgaga atggccagac catgatgaaa taacataagg tggtcccgtc
     2761 ggcaagagac atccacttaa cgtatgcttg caataagtgc gagtgaaagg aatagtattc
     2821 tgagtgtcgt attgagtctg agtgagacag cgatatgatt gttgattaac ccttagcatg
     2881 tccgtggggt ttgaattaac tcataatatt aattagacga aattattttt aaagttttat
     2941 ttttaataat ttgcgagtac gcaaagctag agttgttggt tggcacacca caaatatact
     3001 gttgccgagc acaattccgc tcgacgaagt tcctattctc tagaaagtat aggaacttct
     3061 agggataaca gggtaatggt acctacctag gatatgcggc cgcgtagtgc cccaactggg
     3121 gtaacctttg agttctctca gttgggggcg taggaattct agtatgtatg taagttaata
     3181 aaaccctttt ttggagaatg tagatttaaa aaaacatatt ttttttttat tttttactgc
     3241 actggatatc attgaactta tctgatcagt tttaaattta cttcgatcca agggtatttg
     3301 aagtaccagg ttctttcgat tacctctcac tcaaaatgac attccactca aagtcagcgc
     3361 tgtttgcctc cttctctgtc cacagaaata tcgccgtctc tttcgccgct gcgtccgcta
     3421 tctctttcgc caccgtttgt agcgttacct agcgtcaatg tccgccttca gttgcacttt
     3481 gtcagcggtt tcgtgacgaa gctccaagcg gtttacgcca tcaattaaac acaaagtgct
     3541 gtgccaaaac tcctctcgct tcttattttt gtttgttttt tgagtgattg gggtggtgat
     3601 tggttttggg tgggtaagca ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat
     3661 caggagctat taattcgcgg aggcagcaaa cacccatctg ccgagcatct gaacaatgtg
     3721 agtagtacat gtgcatacat cttaagttca cttgatctat aggaactgcg attgcaacat
     3781 caaattgtct gcggcgtgag aactgcgacc cacaaaaatc ccaaaccgca atcgcacaaa
     3841 caaatagtga cacgaaacag attattctgg tagctgtgct cgctatataa gacaattttt
     3901 aagatcatat catgatcaag acatctaaag gcattcattt tcgactacat tcttttttac
     3961 aaaaaatata acaaccagat attttaagct gatcctagat gcacaaaaaa taaataaaag
     4021 tataaaccta cttcgtagga tacttcgttt tgttcggggt tagatgagca taacgcttgt
     4081 agttgatatt tgagatcccc tatcattgca gggtgacagc ggacgcttcg cagagctgca
     4141 ttaaccaggg cttcgggcag gccaaaaact acggcacgct cctgccaccc agtccgccgg
     4201 aggactccgg ttcagggagc ggccaactag ccgagaacct cacctatgcc tggcacaata
     4261 tggacatctt tggggcggtc aatcagccgg gctccggatg gcggcagctg gtcaaccgga
     4321 cacgcggact attctgcaac gagcgacaca taccggcgcc caggaaacat ttgctcaaga
     4381 acggtgagtt tctattcgca gtcggctgat ctgtgtgaaa tcttaataaa gggtccaatt
     4441 accaatttga aactcagttt gcggcgtggc ctatccgggc gaacttttgg ccgtgatggg
     4501 cagttccggt gccggaaaga cgaccctgct gaatgccctt gcctttcgat cgccgcaggg
     4561 catccaagta tcgccatccg ggatgcgact gctcaatggc caacctgtgg acgccaagga
     4621 gatgcaggcc aggtgcgcct atgtccagca ggatgacctc tttatcggct ccctaacggc
     4681 cagggaacac ctgattttcc aggccatggt gcggatgcca cgacatctga cctatcggca
     4741 gcgagtggcc cgcgtggatc aggtgatcca ggagctttcg ctcagcaaat gtcagcacac
     4801 gatcatcggt gtgcccggca gggtgaaagg tctgtccggc ggagaaagga agcgtctggc
     4861 attcgcctcc gaggcactaa ccgatccgcc gcttctgatc tgcgatgagc ccacctccgg
     4921 actggactca tttaccgccc acagcgtcgt ccaggtgctg aagaagctgt cgcagaaggg
     4981 caagaccgtc atcctgacca ttcatcagcc gtcttccgag ctgtttgagc tctttgacaa
     5041 gatccttctg atggccgagg gcagggtagc tttcttgggc actcccagcg aagccgtcga
     5101 cttcttttcc tagtgagttc gatgtgttta ttaagggtat ctagcattac attacatctc
     5161 aactcctatc cagcgtgggt gcccagtgtc ctaccaacta caatccggcg gacttttacg
     5221 tacaggtgtt ggccgttgtg cccggacggg agatcgagtc ccgtgatcgg atcgccaaga
     5281 tatggcgaca attttgctat tagcaaagta gcccgggata tggagcagtt gttggccacc
     5341 aaaaatttgg agaagccact ggagcagccg gagaatgggt acacctacaa ggccacctgg
     5401 ttcatgcagt tccgggcggt cctgtggcga tcctggctgt cggtgctcaa ggaaccactc
     5461 ctcgtaaaag tgcgacttat tcagacaacg gtgagtggtt ccagtggaaa caaatgatat
     5521 aacgcttaca attcttggaa acaaattcgc tagattttag ttagaattgc ctgattccac
     5581 acccttctta gtttttttca atgagatgta tagtttatag ttttgcagaa aataaataaa
     5641 tttcatttaa ctcgcgaaca tgttgaagat atgaatatta atgagatgcg agtaacattt
     5701 taatttgcag atggttgcca tcttgattgg cctcatcttt ttgggccaac aactcacgca
     5761 agtgggcgtg atgaatatca acggagccat cttcctcttc ctgaccaaca tgacctttca
     5821 aaacgtcttt gccacgataa atgtaagtct tgtttagaat acatttgcat attaataatt
     5881 tactaacttt ctaatgaatc gattcgattt aggtgttcac ctcagagctg ccagttttta
     5941 tgagggaggc ccgaagtcga ctttatcgct gtgacacata ctttctgggc aaaacgattg
     6001 ccgaattacc gctttttctc acagtgccac tggtcttcac ggcgattgcc tatccgatga
     6061 tcggactgcg ggccggagtg ctgcacttct tcaactgcct ggcgctggtc actctggtgg
     6121 ccaatgtgtc aacgtccttc ggatatctaa tatcctgcgc cagctcctcg acctcgatgg
     6181 cgctgtctgt gggtccgccg gttatcatac cattcctgct ctttggcggc ttcttcttga
     6241 actcgggctc ggtgccagta tacctcaaat ggttgtcgta cctctcatgg ttccgttacg
     6301 ccaacgaggg tctgctgatt aaccaatggg cggacgtgga gccgggcgaa attagctgca
     6361 catcgtcgaa caccacgtgc cccagttcgg gcaaggtcat cctggagacg cttaacttct
     6421 ccgccgccga tctgccgctg gactacgtgg gtctggccat tctcatcgtg agcttccggg
     6481 tgctcgcata tctggctcta agacttcggg cccgacgcaa ggagtagccg acatatatcc
     6541 gaaataactg cttgtttttt tttttaccat tattaccatc gtgtttactg tttattgccc
     6601 cctcaaaaag ctaatgtaat tatatttgtg ccaataaaaa caagatatga cctatagaat
     6661 acaagtattt ccccttcgaa catccccaca agtagacttt ggatttgtct tctaaccaaa
     6721 agacttacac acctgcatac cttacatcaa aaactcgttt atcgctacat aaaacaccgg
     6781 gatatatttt ttatatacat acttttcaaa tcgcgcgccc tcttcataat tcacctccac
     6841 cacaccacgt ttcgtagttg ctctttcgct gtctcccacc cgctctccgc aacacattca
     6901 ccttttgttc gacgaccttg gagcgactgt cgttagttcc gcgcgattcg gttcgctcaa
     6961 atggttccga gtggttcatt tcgtctcaat agaaattagt aataaatatt tgtatgtaca
     7021 atttatttgc tccaatatat ttgtatatat ttccctcaca gctatattta ttctaattta
     7081 atattatgac tttttaaggt aattttttgt gacctgttcg gagtgattag cgttacaatt
     7141 tgaactgaaa gtgacatcca gtgtttgttc cttgtgtaga tgcatctacg cccccaactg
     7201 agagaactca aaggttaccc cagttggggc actacgc
//
